New Alliance Dye Chem Pvt. Ltd.
Bile Extract

Bile Extract

Send Inquiry

General Information of Bile Extract

 

Bile salt constitutes pure substances, sodium conjugates of glycotaurocholate, and those are used as selective agents mainly to inhibit gram-positive organisms, non-intestinal organisms and spore formers while permitting the growth of gram-negative enterics. Bile inhibits Enterococcus and Staphylococcus species. 

Specification:-

 

Physical and Chemical parameter-

 

S.No.

Parameters

Specifications

1.

Appearance

Greenish to yellowish colour free flowing powder, no pungent smell.

2.

Solubility (2% soln. at 25oC)

Soluble in distilled water and alcohol.

3.

Clarity (2% Soln. at 121ÂșC.)*

Clear solution. No ppt.

4.

pH  (2% Soln. at 25ÂșC)

6.5 - 8.0

5.

Loss on drying ( at 105ÂșC)

NMT - 5.0%

6.

Total Ash

NMT - 20.0%

7.

Cholic Acid (as Sodium Cholate)

NLT - 45.0%

 

Microbiological parameter-

S.No.

Parameters

Specifications

1.

Total viable count

NMT- 10,000cfu/gm

2.

Escherichia coli

Negative

3.

Salmonella spp.

Negative

4.

Staphylococcus aureus

Negative

5.

Yeast & molds

100/gm

 

Growth promotion test-

Cultural characteristic observed in MacConkey agar with 0.5% Bile salt powder after incubation at 35-37oC.

S.No.

Microorganism

ATCC

GROWTH

Recovery

1.

Staphylococcus aureus

25923

Inhibited

-----------

2.

Escherichia coli

25922

Luxuriant / bile ppt.

>= 50%

3.

Enterococcus faecalis

29212

Fair-Good

>= 50%

4.

Salmonella typhimurium

14028

Luxuriant

>= 50%

 

Packing : 25kg HDPE bag/HDPE drum
STORAGE: Hygroscopic by nature, store the material below 25ÂșC, protect from direct sunlight.